We are pleased to announce that Surface Plasmon Resonance (SPR) services are now available to the research community on the UC Riverside campus and beyond! We have recently acquired a Biacore SPR 1K system, a state-of-the-art instrument capable of providing high-performance, label-free analysis of biomolecular interactions. This instrument enables researchers to measure binding kinetics, affinity, and specificity in real time, offering critical insights into molecular recognition processes.
In addition to the Biacore SPR 1K, our facility also provides access to Biacore 3000 and Biacore X systems for exploratory research and assay development (for collaboration only). Together, these instruments give researchers flexibility to pursue both cutting-edge kinetic characterization and early-stage feasibility studies.
🔹 Key Features of the Biacore SPR 1K:
• High sensitivity and wide dynamic range for studying diverse biomolecular interactions
• Multiplexing capability with parallel flow channels for increased throughput
• Compatible with a variety of sensor chip chemistries (e.g., NTA, CM5, avidin) for flexible assay development
• Reliable kinetic characterization of proteins, nucleic acids, small molecules, lipids, and complex assemblies
• Automated sample handling for reproducible and efficient data collection
🔹 Range of Applications:
• Protein–protein, protein–lipid, and protein–nucleic acid interactions
• Small-molecule drug screening and target validation
• Epitope mapping and antibody characterization
• Complex binding mechanisms and multi-step interaction analysis
🔹Available Chips and Targeted Applications:
• CM5 - Immobilization of molecules (via -NH2, SH, -CHO, -OH, and -COOH groups)
• Streptavidin (SA) - Immobilization of Biotinylated Ligands
• NTA - Immobilization of His-Tagged Ligands
• Protein-A - Immobilization of Antibodies (through FC Region only)
• Protein-G - Immobilization of Antibodies (all human, many mammalian subclasses)
• L1 - Immobilization of Vesicles/Liposomes while retaining bilayer structure (suitable for transmembrane proteins)
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🔹 Why Choose Us?
Our facility provides:
• State-of-the-art instrumentation for high-sensitivity measurements
• Broad application support
• Expert guidance and consultation for experiment design, assay development, and data analysis
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🔹 Key Services:
• Free consultation to discuss your research goals and determine the best experimental approach
• Assay development support to ensure robust and reproducible results
• Grant application support letters – We are happy to provide official letters of support for proposals that include SPR experiments as part of the research plan
For more information and fee structure, please contact
Prof. Jason Cheng (quan.cheng@ucr.edu)
Lab SPR researcher Cole Ebel (cebel001@ucr.edu)
Lab SPR researcher Westley Van Zant (wvan017@ucr.edu)
Lab SPR researcher Victor Hanson (vhans006@ucr.edu)
We look forward to supporting your research with this cutting-edge technology!
